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AIM To optimize the ultrasound-microwave extraction for total flavonoids from Glycyrrhizae Radix et Rhizoma residue by response surface method.METHODS In addition to the index of extraction rate of total flavonoids for evaluation,the microwave power,extraction time,liquid-soild ratio and ethanol concentration were taken into consideration as influencing factors for the extraction optimization by response surface method on the basis of single factor test.RESULTS The optimal conditions,determined to be 125 W for microwave power,32 min for extraction time,28.4 ∶ 1 for liquid-solid ratio,79％ for ethanol concentration,and 300 W for extraction power,could bring forth a 2.59％ extraction rate of total flavonoids.CONCLUSION This stable and reliable method can be used for extracting total flavonoids from Glycyrrhizae Radix et Rhizoma residue.
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Aim To establish depression rat model with kidney-yang deficiency and evaluate the related parameters.Methods A total of 24 adult SD rats were divided into control group,mod-el group and self-healing group by body weight.Model group and self-healing group were injected hydrocortisone for 21 days, meanwhile the control group was injected sodium chloride.After modeling,the related indexs detection were taken out:open-field test,sucrose preference test and forced swim test.Then the orbital blood was taken to detect 5-HT,DA and NE by LC-MS/MS.And liver,kidney,spleen,thymus,adrenal gland,testis and epididymis were taken to calculate organ index.Results Compared with the control group,model rats displayed depres-sion and kidney yang deficiency syndrome such as dry hairs, chills,back arched and so on. Correspondingly, the body weight increased slowly.Immobility in FST was significantly in-creased. Sucrose preference, horizontal scores and vertical scores was significantly decreased.Organ index decreased in liv-er,kidney,spleen,adrenal,testis and epididymis.The level of 5-HT and NE was significantly high.Compared with the model group,5-HT,NE and DA were significantly reduced,5-HT and DA were even lower than those of the control group.Conclusion Depression rat model with kidney-yang deficiency could be in-duced by continuous injection of hydrocortisone.
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Clinically, kidney?yang deficiency is one of the main TCM syndromes in patients with major depressive disorder. Warming yang and reinforcing kidney as a therapeutic measure for the treatment of depressive disorder has a?chieved good clinical curative effect. However, there are some problems on the study of kidney?yang deficiency depression. One of the reasons is that the establishment methods of depression animal model with kidney?yang deficiency are not uni?fied. In order to provide reference and further improve the replication method of depression animal models with kidney?yang deficiency, we systematically collected and analyzed the experiment results published in the past 15 years about the depres?sion animal model with kidney?yang deficiency induced by glucocorticoid hormone drugs and find out their similarities and differences.
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OBJECTIVE:To optimize the extraction technology of oleanolic acid from Elsholtzia bodinieri V.METHODS:The extraction technology was optimized by orthogonal-test using microwave-assisted dynamic extraction method with oleanolic acid as index;And the content of oleanolic acid was determined by TLC-spectrophotometry.RESULTS:The opti-mum extraction condition was described as follows,the microwave power is600W with methanol as extracting solvent,the flow rate was8ml/min and the inner diameter of extraction tube was10mm.CONCLUSIONS:The microwave-assisted dynamic extraction method is simple,safe,rapid,accurate and with high extraction yield.The optimized extraction technology is stable and feasible,and suitable for industrialized production.
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Objective To study the constituents from the roots of Elsholtzia bodinieri. MethodsThe compounds were isolated and purified by silica gel column chromatography and TLC, and their structures were elucidated on the basis of spectroscopic methods and physicochemical properties. ResultsFive phenolic constituents were isolated from the ehtanol extract of E. bodinieri roots and were identified as 3-hydroxy-4′, 5-dimethoxybibenzyl-4-O-?-D-xylopyranosyl-(1→6)-?-D-glucopyranoside (Ⅰ), gallic acid (Ⅱ), (+)-catechin (Ⅲ), vanillin (Ⅳ), kaempferol (Ⅴ), respectively. ConclusionCompound Ⅰ is a new compound named as bodinieriside C.